Journal: Nucleic Acids Research

Article Title: Blastocrithidia nonstop mitochondrial genome and its expression are remarkably insulated from nuclear codon reassignment

doi: 10.1093/nar/gkae168

Figure Lengend Snippet: Electrophoretic analysis of the activity of complex I in mitochondrial lysates of P. serpens and B. nonstop . Mitochondrial proteins were stained by Coomassie Brilliant Blue in 2–10% Native gel (CBB). Complex I was visualized by the in-gel activity assay of NADH dehydrogenase (NDH). The asterisk indicates a position of the missing activity band of complex I in B. nonstop . Sizes of molecular weight marker bands (in kDa) are indicated on the right.

Article Snippet: Total protein content in gels was determined by staining with Coomassie Brilliant Blue (PanReac AppliChem).

Techniques: Activity Assay, Staining, Molecular Weight, Marker

Journal: Scientific Reports

Article Title: (Pro)renin receptor is crucial for Wnt/β-catenin-dependent genesis of pancreatic ductal adenocarcinoma

doi: 10.1038/srep08854

Figure Lengend Snippet: (a) Plasma s(P)RR levels in 20 patients with PDAC and 20 healthy matched controls. Data are mean ± SD values. The boxes encompass the twenty-fifth through seventy-fifth percentiles of results. The lines through the middle of each box represent the medians. The maximum and minimum values within 1.5 × interquartile range (IQR) are shown as whisker caps. Average values are indicated by dots in the boxes. (b) s(P)RR in conditioned medium was assayed for HPDE cells and six different PDAC cell lines. Consistent results were observed when three experiments were repeated. Loading control was determined by CBB staining.

Article Snippet: To obtain a loading control of s(P)RR expression, we used the CBB (Coomassie Brilliant Blue) staining solution (Wako, Osaka, Japan, catalog #17400553) to visualize the protein bands.

Techniques: Whisker Assay, Staining

Journal: Cancers

Article Title: JAK3 Is Expressed in the Nucleus of Malignant T Cells in Cutaneous T Cell Lymphoma (CTCL)

doi: 10.3390/cancers13020280

Figure Lengend Snippet: JAK3 interacts with RNA Polymerase II in MyLa cells. ( a ) Immunoblots after co-immunoprecipitation assay shows JAK3 interacts with POLR2A in MyLa 2000 (up) and MyLa 2059 (down). ( b ) Autoradiograph shows in vitro tyrosine phosphorylation of recombinant human Histone H3 by recombinant human JAK3 (left) and the phosphorylation is inhibited by pre-incubating the kinase, JAK3, with 100 nM JAK inhibitor Tofacitinib citrate (JAKi) for 30 min (right side panel). Coomassie staining of the gel shows Histone H3 in all the samples. Recombinant human JAK2 is used as a positive control for the kinase assay.

Article Snippet: The kinase and the substrate were separated by running samples on 4–20% SDS-PAGE gel (BioRad, Hercules, CA, USA) followed by staining gel with Coomassie Brilliant blue (BioRad, #1610786).

Techniques: Western Blot, Co-Immunoprecipitation Assay, Autoradiography, In Vitro, Phospho-proteomics, Recombinant, Staining, Positive Control, Kinase Assay

Journal: Endocrinology

Article Title: Role of regulator of G protein signaling in desensitization of the glucose-dependent insulinotropic peptide receptor.

doi: 10.1210/endo.139.11.6282

Figure Lengend Snippet: FIG. 7. Immunoblot detection of Gsa in RGS2-HA-transfected L293 cells in the presence (1) or the absence (2) of GIP stimulation. Cell lysates were immunoprecipitated with HA antiserum and resolved on SDS-PAGE gels. The detection of Gsa is shown at the top, and the bottom panel shows a representative of the SDS-PAGE gels stained with Coomassie brilliant blue.

Article Snippet: After electrophoresis, gels were either stained with Coomassie brilliant blue or transferred to a Hybond-C Extra filter (Amersham), and Gsa proteins were detected using Gsa antiserum (Santa Cruz Biotechnology) and the enhanced chemiluminescence system (Amersham) according to the manufacturer’s instruction.

Techniques: Western Blot, Transfection, Immunoprecipitation, SDS Page, Staining